Device

Part:BBa_K4160016

Designed by: Femi Hesen, Wouter Langers, Floor van Boxtel   Group: iGEM22_TU-Eindhoven   (2022-10-09)


STAT-induced SEAP

This composite part encodes for STAT-induced SEAP and is composed of the basic parts STAT promotor (BBa_K4160006) and SEAP (BBa_K1470004).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 209
    Illegal XbaI site found at 233
    Illegal PstI site found at 53
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 209
    Illegal PstI site found at 53
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 209
    Illegal BamHI site found at 227
    Illegal BamHI site found at 459
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 209
    Illegal XbaI site found at 233
    Illegal PstI site found at 53
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 209
    Illegal XbaI site found at 233
    Illegal PstI site found at 53
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 1739
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1100
    Illegal BsaI.rc site found at 1589


Usage & biology

TU-Eindhoven 2022 used this composite part as an output of the Generalized Extracellular Molecule Sensor (GEMS) system that is developed by Scheller et al., 2018.1 Upon activation of the GEMS receptor, STAT-induced SEAP will be expressed and secreted via the JAK/STAT signaling pathway. In the project of TU-Eindhoven 2022, SEAP was used as a genetic reporter protein to investigate the activation of the GEMS receptor (BBa_K4160008, BBa_K4160009, BBa_K4160010, BBa_K4160012, and BBa_K4160013). STAT-induced SEAP was expressed in a pLS13 plasmid.


Characterization

Characterization of STAT-induced SEAP expression by the GEMS system can be found on the BBa_K4160008 page.


References

  1. Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M. Generalized extracellular molecule sensor platform for programming cellular behavior. Nat Chem Biol. Published online 2018. doi:10.1038/s41589-018-0046-z




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